Chemistry Internship Study Questions Section 2

What are the specimen handling requirements for plasma folate analysis?
Protect from light. Heparin tubes
What is the required collection tube for RBC Folate?
EDTA
Are there any pretreatment requirements before running a RBC Folate?
Add lysing reagent to make a hemolysate
What benefits are there to quantitating a RBC Folate rather than a serum/plasma folate?
Serum levels reflect a day-to-date Folate consumption;
RBC Folate reflects a chronic condition
Describe the RBC Morphology associated with B12/Folate Deficiencies
Macrocytic / Megaloblastic
What is the Disease risk associated with Folate Deficiency in Pregnant Women?
Neural Tube Defects
Is there a test which can help determine the risk of Folate Deficiency in Pregnant Women?
AFP on Maternal Serum & Amniotic fluid
Match the Tumor Marker with the Primary Tumor Source:

PSA

Prostate
Match the Tumor Marker with the Primary Tumor Source:

Cortisol

Adrenal
Match the Tumor Marker with the Primary Tumor Source:

CA 125

Ovarian
Match the Tumor Marker with the Primary Tumor Source:

HCG

Choriocarcinoma, Testicular
Match the Tumor Marker with the Primary Tumor Source:

AFP

Hepatocellular / Choriocarcinoma, Testicular
Match the Tumor Marker with the Primary Tumor Source:

CEA

GI, Colon, Lung
Match the Tumor Marker with the Primary Tumor Source:

HE4

Breast
Match the Tumor Marker with the Primary Tumor Source:

CA 15-3

Breast
Match the Tumor Marker with the Primary Tumor Source:

CA 19-9

Pancreatic & Colorectal
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Prostate

PSA
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Adrenal

Cortisol
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Ovarian

CA 125
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Choriocarcinoma, Testicular

HCG
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Hepatocellular / Choriocarcinoma, Testicular

AFP
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

GI, Colon, Lung

CEA
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Breast

HE4 & CA 15-3
Match the Primary Tumor Source with the Tumor Marker that tests for that area:

Pancreatic & Colorectal

CA 19-9
What TISSUE RECEPTOR tests can be done to help determine the BEST COURSE OF TREATMENT for a woman with BREAST CANCER?
Estrogen & Progesterone Receptor
What abnormal conditions cause an accumulation of Lactic acid in the blood?
Tissue hypoxia from shock, Heart disease or Heart attack, Sepsis, Lung Disease, & Severe Crush Injuries
Are there any “NORMAL” Conditions that can cause INCREASED LACTATE?
Exercise
Describe the proper collection tube and handling requirements when testing for Lactate
Gray Top, Put on Ice, Deliver ASAP
What is the “PREDICTIVE” Function of a RISING LACTATE level?
Increased Levels = Increased Mortality
What is the most common test done to assess Fetal Lung Maturity?
Albumin Surfactant Test;
L/S Ratio;
PG
What surfactant serves as a final indicator of Fetal Lung Maturity at the End of Gestation?
PG
What Condition will result in a Newborn who does NOT have sufficient surfactant?
Respiratory Distress Syndrome (RDS)
What is the cause of Hemolytic Disease of the Newborn (HDN)?
Incompatible blood type between mother and baby (usually Rh)
What test can be performed on Amniotic fluid to aid in diagnosing and monitoring HDN?
bilirubin (OD450)
What is the GREATEST Risk to an infant born with HDN?
Anemia & Kericterus
What type of graph can be used to assess the severity of HDN?
Liley Graph;
Zones indicate severity of anemia
Neural Tube Defects (NTD) are associated with Elevated levels of What substance in Maternal Serum and Amniotic Fluid?
Alpha FetoProtein (AFP)
What dietary deficiency has been associated with Neural Tube Defects (NTD)?
Folic Acid/Folate Deficiencies
What cervical swab test serves as an Indicator of Impending Delivery?
Fetal Fibronectin