Chemistry Laboratory 7

Specimens which are suitable for analysis are
serum stored at 2-8°C for 7 days
The therapeutic ranges for salicylate concentration
2-10 mg/dL for analgesia and 10-30 mg/dL for anti-inflammatory.
The toxic ranges
30-60 mg/dL.
The procedure
starts with 4 test tubes with reagent A in each of them. Then add appropriate ingredient to each tube and mix. The initial absorbance is recorded at 340nm, then reagent B is added and mixed and incubated. Final absorbance is measured and change in absorbance is calculated along with concentration of salicylate for each tube.
Why is maintaining a drug level within therapeutic range important?
because an overdose can be deadly, and to beep concentration above minimum effectve concentration so it is still treating the disorder
Three methods for treating salicylate intoxication
activated charcoal, alkaline dieresis and hemodialysis
Clinical manifestations of toxic doses of salicylate
rapid onset of CNS stimulation, sweating, hyperventilation, nausea, flushed skin, ringing in the ears, and fever. chronic overdose can cause renal failure and kidney damage

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